{"id":56,"date":"2005-09-22T21:01:41","date_gmt":"2005-09-23T04:01:41","guid":{"rendered":"http:\/\/outflux.net\/blog\/archives\/2005\/09\/22\/decompiling-myself\/"},"modified":"2005-09-22T21:06:00","modified_gmt":"2005-09-23T04:06:00","slug":"decompiling-myself","status":"publish","type":"post","link":"https:\/\/outflux.net\/blog\/archives\/2005\/09\/22\/decompiling-myself\/","title":{"rendered":"decompiling myself"},"content":{"rendered":"<p>Figured I should try to decompile myself.  The first step would be get a full <a href=\"http:\/\/genome.ucsc.edu\/\">dump<\/a> of my DNA base pairs as letters.  Looks like that&#8217;s <a href=\"http:\/\/en.wikipedia.org\/wiki\/Human_genome\">not going to be easy<\/a> though.  Even a DNA stain takes a <a href=\"http:\/\/www.genelex.com\/paternitytesting\/paternitybook3.html\">lot of steps<\/a> (and I&#8217;ll probably never be allowed to do the radioactive steps myself).  The real goal here is that with current US law, I should copyright myself (I&#8217;m the first performance of the specific base pair &#8220;idea&#8221;) and possibly patent myself (my methods are a unique variation of other methods).<\/p>\n<p>Obviously this doesn&#8217;t take into account my immune system or my memories, but I figure it&#8217;s a good start.  At like just under 10 billion base pairs, that&#8217;s a 10GB program.  I think <a href=\"http:\/\/inkscape.org\/\">Inkscape<\/a> is only 45M or so, and that&#8217;s not even counting shared libraries.<\/p>\n<p>Since I don&#8217;t really want to share my DNA with a company (I&#8217;ve got to be the first to copyright it), I wanted to find out what it would take to sequence at home.  Since a sequencer is in the $100k price range, that&#8217;s not really going to happen.  Talking to my <a href=\"http:\/\/www.nih.gov\/\">NIH<\/a>-employed friend <a href=\"http:\/\/www.livejournal.com\/users\/techne23\/\">techne23<\/a>, she suggested a possible &#8220;cheap&#8221; way to do it would be in pieces, doing <a href=\"http:\/\/en.wikipedia.org\/wiki\/Polymerase_chain_reaction\">PCR<\/a>s on specific <a href=\"http:\/\/en.wikipedia.org\/wiki\/Single_nucleotide_polymorphism\">SNP<\/a>s, and send those out for sequencing to get back base pair letters.  For example, on a gene, the <a href=\"http:\/\/genome.ucsc.edu\/cgi-bin\/hgTracks?position=chr14:79736596-79747602&#038;hgsid=60582885&#038;knownGene=pack&#038;hgFind.matches=BC063118,\">red ones here<\/a> are considered &#8220;interesting&#8221;.  The PCR machines can be <a href=\"http:\/\/www.surpluslab.com\/category_details.aspx?CategoryID=118\">had for cheap<\/a>, too.<\/p>\n<p>So, in summary:<\/p>\n<ul>\n<li>need all the standard lab stuff (centifuge, gloves, tips, pipets, tubes, autoclave, glassware, etc)<\/li>\n<li>need chemicals to isolate my DNA<\/li>\n<li>need a little space in my freezer to store my DNA<\/li>\n<li>need to buy PCR reagents, about $100 for 50-100 reactions<\/li>\n<li>need two base pair-specific primers at $40 total for up to 500 base pairs per PCR<\/li>\n<li>need thermal cycler to do the PCR in<\/li>\n<li>need electrophoresis equipment to see if the PCR worked (maybe reuse my UV EPROM wiper?)<\/li>\n<li>need toxic (careful!) reagents for the electrophoresis<\/li>\n<li>need a sequencing company that is willing to work with a non-University<\/li>\n<li>need FedEx account to ship PCR to sequencers  :)<\/li>\n<\/ul>\n<p>Or I can <a href=\"http:\/\/www.wellcome.ac.uk\/en\/genome\/technologies\/hg17b011.html\">spend crazy money<\/a> doing thousands of SNPs at once in microarrays.  (Or wait until they&#8217;re in every doctor&#8217;s office.)<\/p>\n<p style='text-align:left'>&copy; 2005, <a href=\"https:\/\/outflux.net\/blog\/\">Kees Cook<\/a>. This work is licensed under a <a rel=\"license\" href=\"http:\/\/creativecommons.org\/licenses\/by-sa\/4.0\/\">Creative Commons Attribution-ShareAlike 4.0 License<\/a>.<br \/><a rel=\"license\" href=\"http:\/\/creativecommons.org\/licenses\/by-sa\/4.0\/\"><img decoding=\"async\" alt=\"CC BY-SA 4.0\" style=\"border-width:0\" src=\"https:\/\/i.creativecommons.org\/l\/by-sa\/4.0\/88x31.png\" \/><\/a> <\/p>\n","protected":false},"excerpt":{"rendered":"<p>Figured I should try to decompile myself. The first step would be get a full dump of my DNA base pairs as letters. Looks like that&#8217;s not going to be easy though. Even a DNA stain takes a lot of steps (and I&#8217;ll probably never be allowed to do the radioactive steps myself). The real [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[8,10],"tags":[],"_links":{"self":[{"href":"https:\/\/outflux.net\/blog\/wp-json\/wp\/v2\/posts\/56"}],"collection":[{"href":"https:\/\/outflux.net\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/outflux.net\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/outflux.net\/blog\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/outflux.net\/blog\/wp-json\/wp\/v2\/comments?post=56"}],"version-history":[{"count":0,"href":"https:\/\/outflux.net\/blog\/wp-json\/wp\/v2\/posts\/56\/revisions"}],"wp:attachment":[{"href":"https:\/\/outflux.net\/blog\/wp-json\/wp\/v2\/media?parent=56"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/outflux.net\/blog\/wp-json\/wp\/v2\/categories?post=56"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/outflux.net\/blog\/wp-json\/wp\/v2\/tags?post=56"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}